Bio Lab Essay Example

Bio Lab Essay Example Bio Lab Essay Bio Lab Essay Santiago NevillePeriod 110/14/12 Enzyme lab Enzymes are biological catalysts that speed up the process of chemical reactions. They are also proteins, and most enzymes activities occur within organism. They decrease activation energy, energy that is needed to start a chemical reaction. Enzymes are substrate specific substrates ending in -ase, enzymes ending in -ase. External factors, such as temperature, pH, and concentration of the substrate, affect the enzymes activity in the lab, pectase and cellulase will be used to compare different enzymes on apple sauce. The lab is designed to investigate the effect of different enzymes on apple juice production The combination of pectinase and cellulase will be the most effective simply because two is better than one. MATERIALS: apple sauce, pectinase enzyme, cellulase enzymes, funnels, filter paper, lab aprons, splash-proof goggles, paper towels, stirring rods, graduated cylinders, access to the water, paperclips, access to a stop watch, and access to a triple bean balance. Procedure: 1) Collect materials 2) Follow safety protocol ) Measure 1 tablespoon of apple sauce and place it in a cup 4) Add 10 drops of pectinase to the apple sauce and stir for 1 minute 5) Line a funnel with a coffee filter, place funnel in a graduated cylinder, empty the mixture into the funnel, and let it filter for 5 minutes. 6) Record the amount of juice collected 7) Repeat steps 1-5 with cellulase and water. Enzyme Lab 4 3. 2 3. 1 3. 28 2. 4 1. 6 Cellulase Pectinase C+P Water 0. 8 0. 86 0. 52 Amount of apple juice in ml A mount of apple juice in ml 0 Average Type of Enzyme Conclusion: Enzymes are catalysts that speed up the process of chemical reactions. They are also proteins, and most Enzymatic activities occur within organism. They decrease the activation energy that is needed to start a chemical reaction. The problem was the effect that different enzymes have on apple sauce, and the lab was designed to investigate this. The combination of pectinase and cellulase will be the most effective simply because two is better than one. The hypothesis was correct because it collected the most amount of apple sauce with 3. 28 ml. We tested the enzymes by mixing it with apple sauce. We got our results by seeing which one collected the most apple sauce. The combination of both pectinase and cellulose was the most effective on apple juice production with 3. 28 ml. The water was the least effective with 0. 52 ml of apple juice collected. In the lab we all collaborated to get the most valid results we could get. The results were collected by testing each mixture in the same way and kept all the control variables constant. This experiment is valid because the controlled variables were kept constant. The independent variable was the type of enzyme or mixture we tested. The dependent variable was the amount (in ml) of apple juice that would be collected out of the coffee filter paper. The controlled variables were: to keep the amount of apple sauce the same, the size of the coffee filter paper, amount of each mixture, amount of time spent on mixing it with the apple sauce and the amount of time allowed letting it drop through the filter paper. The experiment taught us that the combination of the two enzymes was the most effective. Santiago Neville Biology Honors period 1